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KMID : 0903520060490030215
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
2006 Volume.49 No. 3 p.215 ~ p.220
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Determination of Optimal Conditions by Response Surface Methodology and Quality Characteristics of Water Extracts of Phellinus linteus
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Youn Sun-Joo
Cho Jun-Gu
Kwoen Dae-Jun
Choi Ung-Kyu
Kang Sun-Chul
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Abstract
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Response surface methodology was implemented to determine an optimal extraction condition in Phellinus linteus water extract. Extraction was performed on 10 experimental conditions including independent variables such as extraction time (1~5 hrs) and water volume over sample (sample : H2O = 1 : 40~200, W/V), color browning, reducing and total sugar, that were based on the significant levels of 10% of central composition design. Color browning, reducing and total sugar contents were found to be more affected when the water volume was increased rather than extraction time. Maximum extraction condition was acquired at extraction time of 3.0~4.5 hrs and water volume of 40~58.2 ml. Being extracted at the optimal extraction condition two of the free sugars, sucrose (0.126%) and glucose (0.012%), were detected. Total content of the free amino acids was found to be 503.26 μg%. Among them, essential amino acid contents were revealed as 5.4%. One major peak from gel permeation chromatography contained polysaccharide(s) with the molecular weights of 10 KDa.
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KEYWORD
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Phellinus linteus, response surface methodology, optimal condition, water extraction
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